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User: daisy1561

Genetics "Stronger" Genetics Extra Credit Video Lyrics: That- that- that promotes me can only make me stronger once i've got dna pol 3 i can get so much longer i know i've got to be right now cuz Pol 1 is never wronger i need ligase to hurry up now cuz fragments just don't belong here.... Let's replicate tonight Be semi-conservative tonight I've got the template and the primer tonight and you brought the dNTP's, right? Awesome, the lagging strand won't be a bore Okazaki fragments are so hard core I ask cuz i'm not sure Does ligase really seal those gaps anymore Bind in the presence of primase and then it gets unzipped by the helicase you should be so honored by gyrase that he would even unwind you anyways so go ahead go nuts let's replicate cuz polymerase has a low error rate 5 prime to 3 never it never deviates except to correct the wrong base take this!! HATERZZZZ!!! :D Tags: Genetics Stronger

User: lalaxscream3

Stony Brook LIGASE field trip :O Tags: ligase stony brook OneTrueMedia

User: FreeScienceLectures

DNA Replication Process http://www.FreeScienceLectures.com We travel inside nucleus to see how the DNA replicates. When DNA replicates its strands are separated by enzine helicase. Single-stranded DNA binding proteines keep the strands from (...?). One DNA strand encodes the leading strand using DNA Polymerase III. Just watch to see what is going on. --- It's Never too Late to Study: http://www.FreeScienceLectures.com --- Notice: This video is copyright by its respectful owners. The website address on the video does not mean anything. --- Tags: dna replication enzine helicase strand polymerase encode okazaki fragments rna primase primer ligase

User: qc0323

DNA Replication Song Biology Project (Just in case you didn't know... We worked on this at like 4am in the morning... yes..yes... LAST MINUTE project!! but turned out really well!!) Lyrics: Deox / y-ribo / nuclei / ic-acid / is made from two strands, a double helix So then, the strands come apart, Nitrogen bases, they start to unzip By DNA helicase H2 bonds Between adenine and thymine H2 bonds Between cytosine and guanine H2 bonds The ladder structure is strongest in Phosphodiester links D-N / A Pol-ym / er-ase Builds the new strand From the / 5" to 3" Direction H2 bonds Between adenine and thymine H2 bonds Between cytosine and guanine H2 bonds The ladder structure is strongest in Phosphodiester links Now I can see that its replicating In a way thats quite interesting (YEAH!) No matter the distance, the leading strand Is followed by the lagging one Finally...the ligase Seals up all the breaks A new....a new....a new....DNNNNNNNNNNA! And it starts again!! Oh, how I love bi-olo-gy (yeah yeah yeah) It's better than chemistry And next time I have to go pee (I know) what's happening inside of me Tags: DNA

User: beriwel

wirman en cotorra pt.2 tu sabe por ligase Tags: wirman en cotorra pt.2

User: szeatam

Original Biotech Rap (R. enzymes) skip first 20sec! Before there was notorious G.F.P. There was Biotech Nation. Straight out of da T.dot Murder High biatch PLEASE SKIP THE FIRST 20SEC OF BLACKSCREEN!! R.Enzyme Lyrics: For some people it is quite hard to tell/ The roles of enzymes in bacterial cells/ It's used for protection to stop the invasion/ of foreign vexation DNA infestation/ It used to be a fact of life that we would run/ When viral phages would prey on our young/ When one lives by the hour never think about the days/ When our population will never reach stationary phase/ The restriction enzymes searches the grand genome/ Cuts the DNA sites arranged in pallindromes/ Cleaving the bonds in the right location Yielding 'sticky' ends in the final conclusion Restrictions enzymes have different categories/ Type I and III got no specificity/ Cutting here and there sometimes quite randomly/ This is how it works, yo im talking seriously/ Type II enzymes work differently/ Cutting DNA with amazing accuracy/ This is what differs these identities/ Type II is useful not type I or III Yo wait wait wait wait...I don't think you understand how serious this stuff is. (hahaha) You mallidrome, show this kid who's boss. Yea. Lay it down. Yo man my rhymes will leave you scattered in pieces/ Like DNA fragments through electrophoresis/ I come to my thesis based on your gel reading/ Fragment position due to number of base pairings/ Your DNA is cut-up but don't be afraid/ Yo Jackteria has an enzyme called ligase/ An enzyme that will definitely heal you uppp/ Every single time you go out to hit them uppp/ Biotech is a teaching of the future not the past/ This stuff can be a mystery but I must stay on task/ I am always watching the sky in hopes to unmask/ Always waiting for an answer every time I ask.../ Chorus: (What's biotech) when it has no representation?/ (What's biotech) lords of creation the biotech nation/ (What's biotech) Playing with life based on our life based on our own vision/ (What's biotech)We will for sure face eternal damnation!/ Yea I don't know myself man. You know sometimes you ask yourself. What is biotech? Who can answer that you know? Not me...I know that. They think they so hot injecting foreign DNA/ Ill shank them at their palindrome TGATCA/ These restriction enzymes can't possibly touch me/ I got methylation, yea CH3/ whoo Yo this right here is truly history in the making. See methylation is the modification of DNA/ It screws the restriction enzymes recognition leaving it no way/ Structural modification leads to R. enzyme confusion/ I'm tough but I thank one thing for my cozy condition/ And that's my man SAM, S-adenosine-methodine/ It's the source of methyl, definitely one of a kind/ SAM is quite the unstable guy it aint a fable/ When it comes to bringing methyl to the table it's quite able/ Yep, It's time for biotech man, restriction enzymes got all the time in the world, give it a twirl,, let's goo biotech, feel the flow, yeah yeah I'm feeling it man, yo you know what I'm saying, straight up, biotech. That's our life yo, what can I say. Yea yea I know what you guys are saying, I can feel it too. It's a vibe, it's going all through you. You can't see, it's all there, it's biotech yo yo yo I know what you be talking about, yo, wor- word up Now I hope you learn something from this presentation/ A creative representation for the biotech population/ (Chorus) nation..nation...nation....nation........ Tags: asian biotech rap notorious enzymes nerdcore biotechnology Andrew Tam Tom Mallin Jimmy Le Jack Huang de bu ai wilber

User: Biologisjefen

Overføring av DNA til en bakterie 3BI elevøvelse Tags: Biologi DNA bakterie Ecori ligase lipase virus

User: undynnite

Ubiquinone Freestyle... you know...? Sorry, I'm not l337 enough to sync music up with the video. Oh well. My iPod also died near the end so I had no idea what was going on... Tags: E3 Ligase

User: anicola2

Infection of EYFP-PML I HF cells by vECFP-ICP4 HSV1 Time course of infection of human fibroblasts expressing an EYFP-PML I fusion under the control of the weak gD promoter by the modified HSV-1 virus vECFP-ICP4, whose immediate early protein ICP4 has been coupled with ECFP. The colors are artificial ones, so ECFP is green and EYFP is red. One shot was taken every 5 minutes, so the real length of the events seen here is actually 6 h 30. Initially, ND10 bodies can be seen in most cells' nuclei as red foci (though the exposition is so short that for a minority of cells that only faintly express EYFP-PML I no signal is detected). The initial weak and diffuse green signal corresponds to non specific background (though colour information is lost on these images, which are the result of the merging of two black and white files, it was clear under the microscope's binoculars that this was a different color than ECFP). After about 1 h 30, the red signal begins to weaken in infected cells, almost fully vanishing after about 2 h 30, as HSV-1 immediate early protein ICP0 triggers the proteasome dependent degradation of PML through its E3 ubiquitin ligase activity, thus disrupting ND10 bodies. Conversely, a diffuse green signal starts to appear in those cells, and shortly after several big green dots appear in each infected nucleus: they correspond to ICP4 accumulations in viral replication compartments. The compartments keep growing until the end of the time course experiment. Over the same lapse of time, as expected uninfected cells retain normal looking ND10 and don't develop an ECFP signal. (C) my chief in Glasgow, RD Everett, MRC Virology Unit, Glasgow, with permission to upload this video of one of my experiments in his lab since PML degradation by ICP0 has been an established fact for quite a long time. Tags: HSV PML ND10 ICP4 ICP0 ECFP EYFP CFP YFP infection herpes simplex Everett Roger

User: ligase888

20070602王宏恩+范瑋琪(留言在河岸) 抬槓~ Tags: 比冷

User: monty2222002

Re: DNA Replication Process Video Cam Direct Upload Tags: dna replication enzine helicase strand polymerase encode okazaki fragments rna primase primer ligase

User: remyfl

Proteosomes Proteasomes are large protein complexes inside all eukaryotes and archaea, as well as in some bacteria. In eukaryotes, they are located in the nucleus and the cytoplasm.The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. Enzymes that carry out such reactions are called proteases. Proteasomes are a major mechanism by which cells regulate the concentration of particular proteins and degrade misfolded proteins. The degradation process yields peptides of about seven to eight amino acids long, which can then be further degraded into amino acids and used in synthesizing new proteins.Proteins are tagged for degradation by a small protein called ubiquitin. The tagging reaction is catalyzed by enzymes called ubiquitin ligases. Once a protein is tagged with a single ubiquitin molecule, this is a signal to other ligases to attach additional ubiquitin molecules. The result is a polyubiquitin chain that is bound by the proteasome, allowing it to degrade the tagged protein Tags: Cell protein complex eukaryotes amino acids cell nucleus

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